TY - JOUR
T1 - Identification and evaluation of universal epitopes in Plasmodium vivax Duffy binding protein
AU - Saravia, Carolina
AU - Martinez, Paola
AU - Granados, Diana S.
AU - Lopez, Carolina
AU - Reyes, Claudia
AU - Patarroyo, Manuel A.
N1 - Funding Information:
Special gratitude goes to Professor Manuel Elkin Patarroyo for all his valuable comments. This research was supported by the ‘‘Instituto Colombiano para el Desarrollo de la Ciencia ‘Francisco Jose de Caldas’ (COLCIENCIAS)” contract RC-2008. Nora Martinez patiently helped translating the manuscript and Alvaro Mongui helped with the artwork.
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2008/12/26
Y1 - 2008/12/26
N2 - Selected PvDBP-derived synthetic peptides were tested in competition assays with HLA molecules in order to identify and evaluate their binding to a wide range of MHC class II molecules. Binding was evaluated as the peptide's ability to displace the biotinylated control peptide (HA306-318) and was detected by a conventional ELISA. Thus, one epitope for the HLA-DR1 molecule, two epitopes for the HLA-DR4 molecule, six epitopes for the HLA-DR7 molecule and three epitopes for the HLA-DR11 molecule displaying a high binding percentage (above 50%) were experimentally obtained. The in vitro results were compared with the epitope prediction results. Two peptides behaved as universal epitopes since they bound to a larger number of HLA-DR molecules. Given that these peptides are located in the conserved PvDBP region II, they could be considered good candidates to be included in the design of a synthetic vaccine against Plasmodium vivax malaria.
AB - Selected PvDBP-derived synthetic peptides were tested in competition assays with HLA molecules in order to identify and evaluate their binding to a wide range of MHC class II molecules. Binding was evaluated as the peptide's ability to displace the biotinylated control peptide (HA306-318) and was detected by a conventional ELISA. Thus, one epitope for the HLA-DR1 molecule, two epitopes for the HLA-DR4 molecule, six epitopes for the HLA-DR7 molecule and three epitopes for the HLA-DR11 molecule displaying a high binding percentage (above 50%) were experimentally obtained. The in vitro results were compared with the epitope prediction results. Two peptides behaved as universal epitopes since they bound to a larger number of HLA-DR molecules. Given that these peptides are located in the conserved PvDBP region II, they could be considered good candidates to be included in the design of a synthetic vaccine against Plasmodium vivax malaria.
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U2 - 10.1016/j.bbrc.2008.10.153
DO - 10.1016/j.bbrc.2008.10.153
M3 - Research Article
C2 - 19000655
AN - SCOPUS:56349138132
SN - 0006-291X
VL - 377
SP - 1279
EP - 1283
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -
