Identification and evaluation of universal epitopes in Plasmodium vivax Duffy binding protein

Carolina Saravia, Paola Martinez, Diana S. Granados, Carolina Lopez, Claudia Reyes, Manuel A. Patarroyo

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Selected PvDBP-derived synthetic peptides were tested in competition assays with HLA molecules in order to identify and evaluate their binding to a wide range of MHC class II molecules. Binding was evaluated as the peptide's ability to displace the biotinylated control peptide (HA306-318) and was detected by a conventional ELISA. Thus, one epitope for the HLA-DR1 molecule, two epitopes for the HLA-DR4 molecule, six epitopes for the HLA-DR7 molecule and three epitopes for the HLA-DR11 molecule displaying a high binding percentage (above 50%) were experimentally obtained. The in vitro results were compared with the epitope prediction results. Two peptides behaved as universal epitopes since they bound to a larger number of HLA-DR molecules. Given that these peptides are located in the conserved PvDBP region II, they could be considered good candidates to be included in the design of a synthetic vaccine against Plasmodium vivax malaria.

Original languageEnglish (US)
Pages (from-to)1279-1283
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume377
Issue number4
DOIs
StatePublished - Dec 26 2008

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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