Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein

Luisa Matheus; G. Eric Blair

doi:10.1016/j.bbaexp.2003.08.010

Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein

Luisa Matheus, G. Eric Blair

Research output: Contribution to journal › Research Article › peer-review

Abstract

The myelin basic proteins (MBPs) are the major proteins of the myelin membrane. Multiple MBP mRNAs and protein isoforms are generated by alternative RNA splicing. Here we describe the isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform from the rat (Rattus norvegicus). The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. RT-PCR analysis of brain mRNA showed that transcripts encoding the 17-kDa isoform were expressed at higher levels early in post-natal development, up to 7 days post-partum.

Original language	English (US)
Pages (from-to)	47-53
Number of pages	7
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1630
Issue number	1
DOIs	https://doi.org/10.1016/j.bbaexp.2003.08.010
State	Published - Oct 20 2003
Externally published	Yes

All Science Journal Classification (ASJC) codes

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/j.bbaexp.2003.08.010

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title = "Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein",

abstract = "The myelin basic proteins (MBPs) are the major proteins of the myelin membrane. Multiple MBP mRNAs and protein isoforms are generated by alternative RNA splicing. Here we describe the isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform from the rat (Rattus norvegicus). The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. RT-PCR analysis of brain mRNA showed that transcripts encoding the 17-kDa isoform were expressed at higher levels early in post-natal development, up to 7 days post-partum.",

author = "Luisa Matheus and Blair, {G. Eric}",

note = "Funding Information: This research was funded by the Multiple Sclerosis Society of Great Britain and Northern Ireland. We thank Dr. A. Asipu for helpful advice and discussion. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2003",

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T1 - Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein

AU - Matheus, Luisa

AU - Blair, G. Eric

N1 - Funding Information: This research was funded by the Multiple Sclerosis Society of Great Britain and Northern Ireland. We thank Dr. A. Asipu for helpful advice and discussion. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2003/10/20

Y1 - 2003/10/20

N2 - The myelin basic proteins (MBPs) are the major proteins of the myelin membrane. Multiple MBP mRNAs and protein isoforms are generated by alternative RNA splicing. Here we describe the isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform from the rat (Rattus norvegicus). The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. RT-PCR analysis of brain mRNA showed that transcripts encoding the 17-kDa isoform were expressed at higher levels early in post-natal development, up to 7 days post-partum.

AB - The myelin basic proteins (MBPs) are the major proteins of the myelin membrane. Multiple MBP mRNAs and protein isoforms are generated by alternative RNA splicing. Here we describe the isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform from the rat (Rattus norvegicus). The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. RT-PCR analysis of brain mRNA showed that transcripts encoding the 17-kDa isoform were expressed at higher levels early in post-natal development, up to 7 days post-partum.

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

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Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein

Abstract

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