Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein

Luisa Matheus, G. Eric Blair

Research output: Contribution to journalArticlepeer-review

Abstract

The myelin basic proteins (MBPs) are the major proteins of the myelin membrane. Multiple MBP mRNAs and protein isoforms are generated by alternative RNA splicing. Here we describe the isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform from the rat (Rattus norvegicus). The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. RT-PCR analysis of brain mRNA showed that transcripts encoding the 17-kDa isoform were expressed at higher levels early in post-natal development, up to 7 days post-partum.

Original languageEnglish (US)
Pages (from-to)47-53
Number of pages7
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1630
Issue number1
DOIs
StatePublished - Oct 20 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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