Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease

Glaucia Diniz Alessio, Fernanda Fortes de Araújo, Jéssica Spínola Silva, Policarpo Ademar Sales Júnior, Matheus de Souza Gomes, Laurence Rodrigues do Amaral, Juan David Ramírez, Carolina Flórez, Andréa Teixeira-Carvalho, Melina de Barros Pinheiro, Marta de Lana, Olindo Assis Martins-Filho

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Abstract

The molecular and serological methods available for Discrete Typing Units (DTU)-specific diagnosis of Trypanosoma cruzi in chronic Chagas disease present limitations. The study evaluated the performance of Human Chagas-Flow ATE-IgG1 for universal and DTU-specific diagnosis of Chagas disease. A total of 102 sera from Chagas disease patients (CH) chronically infected with TcI, TcVI or TcII DTUs were tested for IgG1 reactivity to amastigote/(A), trypomastigote/(T) and epimastigote/(E) antigens along the titration curve (1:250–1:32,000). The results demonstrated that “AI 250/40%”, “EVI 250/30%”, “AII 250/40%”, “TII 250/40%” and “EII 250/30%” have outstanding accuracy (100%) to segregate CH from non-infected controls. The attributes “TI 4,000/50%”, “EI 2,000/50%”, “AVI 8,000/60%” and “TVI 4,000/50%” were selected for DTU-specific serotyping of Chagas disease. The isolated use of “EI 2,000/50%” provided the highest co-positivity for TcI patients (91%). The combined decision tree algorithms using the pre-defined sets of attributes showed outstanding full accuracy (92% and 97%) to discriminate “TcI vs TcVI vs TcII” and “TcI vs TcII” prototypes, respectively. The elevated performance of Human Chagas-Flow ATE-IgG1 qualifies its use for universal and TcI/TcVI/TcII-specific diagnosis of Chagas disease. These findings further support the application of this method in epidemiological surveys, post-therapeutic monitoring and clinical outcome follow-ups for Chagas disease.

Translated title of the contributionChagas-Flow humano ATE-IgG1 para el serodiagnóstico universal avanzado y específico de Trypanosoma cruzi
Original languageEnglish (US)
Article number13296
Pages (from-to)1-14
Number of pages14
JournalScientific Reports
Volume10
Issue number1
DOIs
StatePublished - Aug 6 2020

All Science Journal Classification (ASJC) codes

  • General

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