Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil

Maria F. Villegas-Torres, Oscar C. Bedoya-Reina, Camilo Salazar, Martha J. Vives-Florez, Jenny Dussan

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

The study of recent evolution of the arsC genes amplified from microorganisms inhabiting a Colombian oil-polluted soil with high concentrations of arsenic was performed through the isolation of 26 bacterial morphotypes resistant to 10. mM of arsenate. A 353. bp fragment of the gene coding for arsenate-reductase enzyme (i.e. arsC), and a 500. bp 16S rDNA partial sequence were sequenced for 16 morphotypes of the 26 previously isolated. arsC sequences clustered on the same clade with previously reported arsC chromosomal genes of Escherichia coli and Shigella sp.; while 16S rDNA sequences grouped within the genus Pseudomonas and Bacillus. The GC content and the Codon Adaptation Index (CAI) were calculated and statistically compared, both supported the previous results. The Isolation-Migration model (IM model) was applied to calculate the genetic flux between each clade defined by the phylogenetic analysis. In general, the existence of recent horizontal gene transfer (HGT) events was confirmed, and the presence of the arsC gene in Bacillus sphaericus is reported for the first time.

Original languageEnglish (US)
Pages (from-to)147-152
Number of pages6
JournalInternational Biodeterioration and Biodegradation
Volume65
Issue number1
DOIs
StatePublished - Jan 2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biomaterials
  • Waste Management and Disposal

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