TY - JOUR
T1 - Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil
AU - Villegas-Torres, Maria F.
AU - Bedoya-Reina, Oscar C.
AU - Salazar, Camilo
AU - Vives-Florez, Martha J.
AU - Dussan, Jenny
N1 - Funding Information:
This work was funded by the Research Fund from de Science Faculty of Universidad de los Andes, Colombia. We are thankful for the samples facilitated by Hugo Vladimir Ramirez, the strain Bacillus subtilis 168 donated by Dr. Tsutomu Sato (Agriculture University of Tokyo, Japan), and for correction of the document by Maria Alejandra Bautista. CS was funded by COLCIENCIAS grant 7155-CO .
PY - 2011/1
Y1 - 2011/1
N2 - The study of recent evolution of the arsC genes amplified from microorganisms inhabiting a Colombian oil-polluted soil with high concentrations of arsenic was performed through the isolation of 26 bacterial morphotypes resistant to 10. mM of arsenate. A 353. bp fragment of the gene coding for arsenate-reductase enzyme (i.e. arsC), and a 500. bp 16S rDNA partial sequence were sequenced for 16 morphotypes of the 26 previously isolated. arsC sequences clustered on the same clade with previously reported arsC chromosomal genes of Escherichia coli and Shigella sp.; while 16S rDNA sequences grouped within the genus Pseudomonas and Bacillus. The GC content and the Codon Adaptation Index (CAI) were calculated and statistically compared, both supported the previous results. The Isolation-Migration model (IM model) was applied to calculate the genetic flux between each clade defined by the phylogenetic analysis. In general, the existence of recent horizontal gene transfer (HGT) events was confirmed, and the presence of the arsC gene in Bacillus sphaericus is reported for the first time.
AB - The study of recent evolution of the arsC genes amplified from microorganisms inhabiting a Colombian oil-polluted soil with high concentrations of arsenic was performed through the isolation of 26 bacterial morphotypes resistant to 10. mM of arsenate. A 353. bp fragment of the gene coding for arsenate-reductase enzyme (i.e. arsC), and a 500. bp 16S rDNA partial sequence were sequenced for 16 morphotypes of the 26 previously isolated. arsC sequences clustered on the same clade with previously reported arsC chromosomal genes of Escherichia coli and Shigella sp.; while 16S rDNA sequences grouped within the genus Pseudomonas and Bacillus. The GC content and the Codon Adaptation Index (CAI) were calculated and statistically compared, both supported the previous results. The Isolation-Migration model (IM model) was applied to calculate the genetic flux between each clade defined by the phylogenetic analysis. In general, the existence of recent horizontal gene transfer (HGT) events was confirmed, and the presence of the arsC gene in Bacillus sphaericus is reported for the first time.
UR - http://www.scopus.com/inward/record.url?scp=78650678167&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650678167&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2010.10.007
DO - 10.1016/j.ibiod.2010.10.007
M3 - Article
AN - SCOPUS:78650678167
SN - 0964-8305
VL - 65
SP - 147
EP - 152
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
IS - 1
ER -