Genetic Variations in the Purinergic P2X7 Receptor Are Associated with the Immune Response to Ocular Toxoplasmosis in Colombia

Carlos A. Naranjo-Galvis, Rima McLeod, Jorge Enrique Gómez-Marín, Alejandra de-la-Torre, Cristian Rocha-Roa, Néstor Cardona, Juan Carlos Sepúlveda-Arias

Research output: Contribution to journalArticlepeer-review

Abstract

Ocular toxoplasmosis (OT) is characterized by inflammation within the eye and is the most recognized clinical manifestation of toxoplasmosis. The objective of this study was to identify new single-nucleotide polymorphisms (SNPs) in the P2RX7 gene that may have significance in the immune response to OT in Colombian patients. A case–control study was conducted to investigate the associations between SNPs (rs1718119 and rs2230912) in the P2RX7 gene and OT in 64 Colombian patients with OT and 64 controls. Capillary electrophoresis was used to analyze the amplification products, and in silico algorithms were employed to predict deleterious SNPs. Stability analysis of amino acid changes indicated that both mutations could lead to decreased protein structure stability. A nonsynonymous SNP, Gln460Arg, located in the long cytoplasmic tail of the receptor, showed a significant association with OT (Bonferroni correction (BONF) = 0.029; odds ratio OR = 3.46; confidence interval CI: 1.05 to 11.39), while no significant association between rs1718119 and OT risk was observed. Based on the 3D structure analysis of the P2RX7 protein trimer, it is hypothesized that an increase in the flexibility of the cytoplasmic domain of this receptor could alter its function. This SNP could potentially serve as a biomarker for identifying Colombian patients at risk of OT.

Translated title of the contributionVariaciones genéticas en el receptor purinérgico P2X7 se asocian con la respuesta inmune a la toxoplasmosis ocular en Colombia
Original languageEnglish (US)
Article number2508
JournalMicroorganisms
Volume11
Issue number10
DOIs
StatePublished - Oct 7 2023

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)
  • Virology

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