TY - JOUR
T1 - Genetic diversity of the entomopathogenic fungus Cordyceps tenuipes in forests and butterfly gardens in Quindío, Colombia
AU - Castillo, Leidy P.
AU - Osorio, Alejandro
AU - Vargas, Natalia
AU - Sanjuan, Tatiana
AU - Grajales, Alejandro
AU - Restrepo, Silvia
N1 - Funding Information:
This work was supported by the Mycology and Phytopathology Laboratory of the Universidad de los Andes . We thank Diana Giraldo from Amaranta Butterfly Garden and the Butterfly Garden from Quindío Botanical Garden for making collections available for this study. This research was partially financed by Center for Analytical Chemistry and Microbiology , Institute for Microbiology and Biochemistry Hochschule Geisenheim University – Germany (Grant IB-KOL-2015-020- COLCIENCIAS 702-2015 ) in head of Andreas Leclerque.
Funding Information:
This work was supported by the Mycology and Phytopathology Laboratory of the Universidad de los Andes. We thank Diana Giraldo from Amaranta Butterfly Garden and the Butterfly Garden from Quind?o Botanical Garden for making collections available for this study. This research was partially financed by Center for Analytical Chemistry and Microbiology, Institute for Microbiology and Biochemistry Hochschule Geisenheim University ? Germany (Grant IB-KOL-2015-020- COLCIENCIAS 702-2015) in head of Andreas Leclerque.
Publisher Copyright:
© 2018 British Mycological Society
PY - 2018/9
Y1 - 2018/9
N2 - Cordyceps tenuipes is an entomopathogenic fungus that infects mostly pupae of several lepidopteran families. In Colombia the species has been reported in non-disturbed tropical rain forests and more recently in butterfly gardens. The aim of this study was to assess the genetic diversity in populations of C. tenuipes present in natural (forests) and artificial (e.g. butterfly gardens) environments in the department of Quindío, Colombia, using three molecular nuclear markers ITS, TEF-1α and RPB1. All the samples evaluated corresponded morphologically and phylogenetically to C. tenuipes. The butterfly garden of Quindio Botanical Garden (QBG) showed the highest genetic diversity among all sampling localities and was very similar to that of its adjacent forest. The Amaranta Butterfly Garden (ABG), located north of QBG, showed lower genetic diversity as well as little genetic differentiation with QBG, consistent with the hypothesis of a pathogen transfer from QBG to ABG. Higher FST values were observed for TEF-1α and ITS, revealing genetic differentiation between all demes and the southern forest population. Our research constitutes the first study of the intraspecific diversity of C. tenuipes in Colombia and can serve as the first step in identifying diversity reservoirs and management of epizootic episodes caused by this fungal species.
AB - Cordyceps tenuipes is an entomopathogenic fungus that infects mostly pupae of several lepidopteran families. In Colombia the species has been reported in non-disturbed tropical rain forests and more recently in butterfly gardens. The aim of this study was to assess the genetic diversity in populations of C. tenuipes present in natural (forests) and artificial (e.g. butterfly gardens) environments in the department of Quindío, Colombia, using three molecular nuclear markers ITS, TEF-1α and RPB1. All the samples evaluated corresponded morphologically and phylogenetically to C. tenuipes. The butterfly garden of Quindio Botanical Garden (QBG) showed the highest genetic diversity among all sampling localities and was very similar to that of its adjacent forest. The Amaranta Butterfly Garden (ABG), located north of QBG, showed lower genetic diversity as well as little genetic differentiation with QBG, consistent with the hypothesis of a pathogen transfer from QBG to ABG. Higher FST values were observed for TEF-1α and ITS, revealing genetic differentiation between all demes and the southern forest population. Our research constitutes the first study of the intraspecific diversity of C. tenuipes in Colombia and can serve as the first step in identifying diversity reservoirs and management of epizootic episodes caused by this fungal species.
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U2 - 10.1016/j.funbio.2018.05.003
DO - 10.1016/j.funbio.2018.05.003
M3 - Research Article
C2 - 30115323
AN - SCOPUS:85047996207
SN - 1878-6146
VL - 122
SP - 891
EP - 899
JO - Fungal Biology
JF - Fungal Biology
IS - 9
ER -
