TY - JOUR
T1 - Functional Analysis of BRCA1 3’UTR Variants Predisposing to Breast Cancer
AU - Sierra-Díaz, Diana Carolina
AU - Cabrera, Rodrigo
AU - Gonzalez-Vasquez, Laura Alejandra
AU - Angulo-Aguado, Mariana
AU - Llinás-Caballero, Kevin
AU - Fonseca-Mendoza, Dora Janeth
AU - Contreras-Bravo, Nora Constanza
AU - Restrepo, Carlos Martin
AU - Ortega-Recalde, Oscar
AU - Morel, Adrien
N1 - Publisher Copyright:
© 2024 Sierra-Díaz et al.
PY - 2024
Y1 - 2024
N2 - Purpose: Breast Cancer (BC) is the main female cancer diagnosed worldwide, and it has been described that few genes, such as BRCA1, have a high penetrance for this type of cancer. In this manuscript, we were interested in evaluating the effect of 3’UTR variants on BRCA1 expression. Patients and Methods: To accomplish this objective, Whole Exome Sequencing (WES) data of 400 patients with unselected BC was used to filter variants located in the region of interest of BRCA1 gene, finding two of them (c.*36C>G and c.*369_373del). miRGate and miRanda in silico tools were used to predict microRNA (miRNA) interaction. Results: The two variants (c.*36C>G, c.*369_373del) were predicted to affect miRNA interaction. After cloning of BRCA1 3’UTR into pMIR-Report vector, the construct was transfected into two BC cell lines (MDA-MB-231 and MCF-7), and the variant c.*36C>G evidenced overexpression of reporter gene luciferase, showing that the transcript was not being degraded by the miRNA in MDA-MB-231 cells. Conclusion: The variant seems to protect against Triple Negative BC probably due to the expression level of miRNA in this particular cell line (MDA-MB-231). This is consistent with the clinical history of the patients who harbor BC Hormone Receptors positive (HR+).
AB - Purpose: Breast Cancer (BC) is the main female cancer diagnosed worldwide, and it has been described that few genes, such as BRCA1, have a high penetrance for this type of cancer. In this manuscript, we were interested in evaluating the effect of 3’UTR variants on BRCA1 expression. Patients and Methods: To accomplish this objective, Whole Exome Sequencing (WES) data of 400 patients with unselected BC was used to filter variants located in the region of interest of BRCA1 gene, finding two of them (c.*36C>G and c.*369_373del). miRGate and miRanda in silico tools were used to predict microRNA (miRNA) interaction. Results: The two variants (c.*36C>G, c.*369_373del) were predicted to affect miRNA interaction. After cloning of BRCA1 3’UTR into pMIR-Report vector, the construct was transfected into two BC cell lines (MDA-MB-231 and MCF-7), and the variant c.*36C>G evidenced overexpression of reporter gene luciferase, showing that the transcript was not being degraded by the miRNA in MDA-MB-231 cells. Conclusion: The variant seems to protect against Triple Negative BC probably due to the expression level of miRNA in this particular cell line (MDA-MB-231). This is consistent with the clinical history of the patients who harbor BC Hormone Receptors positive (HR+).
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U2 - 10.2147/TACG.S444546
DO - 10.2147/TACG.S444546
M3 - Research Article
C2 - 38803352
AN - SCOPUS:85195147593
SN - 1178-704X
VL - 17
SP - 57
EP - 62
JO - Application of Clinical Genetics
JF - Application of Clinical Genetics
ER -