Evaluation of the diagnostic performance of nine commercial RT-PCR kits for the detection of SARS-CoV-2 in Colombia

Carolina Hernández; Carolina Florez; Sergio Castañeda; Nathalia Ballesteros; David Martínez; Adriana Castillo; Marina Muñoz; Sergio Gomez; Angelica Rico; Liseth Pardo; Alberto Paniz-Mondolfi; Juan David Ramírez

doi:10.1002/jmv.27051

Evaluation of the diagnostic performance of nine commercial RT-PCR kits for the detection of SARS-CoV-2 in Colombia

Carolina Hernández
, Carolina Florez
, Sergio Castañeda
, Nathalia Ballesteros
, David Martínez
, Adriana Castillo
, Marina Muñoz
, Sergio Gomez
, Angelica Rico
, Liseth Pardo
, Alberto Paniz-Mondolfi
, Juan David Ramírez

Research output: Contribution to Journal › Research Article › peer-review

12 Scopus citations

Abstract

The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has led to the design and development of multiple reverse-transcription polymerase chain reaction kits aimed to facilitate the rapid scale-up of molecular testing for massive screening. We evaluated the diagnostic performance of nine commercial kits, which showed optimal performance and high discriminatory power. However, we observed differences in terms of sensitivity, specificity, and E gene Ct Values and discuss these results in light of the influence of SARS-CoV-2 genetic variability and its potential impact in current molecular diagnostic assays.

Original language	English (US)
Journal	Journal of Medical Virology
DOIs	https://doi.org/10.1002/jmv.27051
State	Accepted/In press - 2021

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Virology
Infectious Diseases

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10.1002/jmv.27051

Cite this

Hernández, C., Florez, C., Castañeda, S., Ballesteros, N., Martínez, D., Castillo, A., Muñoz, M., Gomez, S., Rico, A., Pardo, L., Paniz-Mondolfi, A., & Ramírez, J. D. (Accepted/In press). Evaluation of the diagnostic performance of nine commercial RT-PCR kits for the detection of SARS-CoV-2 in Colombia. Journal of Medical Virology. https://doi.org/10.1002/jmv.27051

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abstract = "The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has led to the design and development of multiple reverse-transcription polymerase chain reaction kits aimed to facilitate the rapid scale-up of molecular testing for massive screening. We evaluated the diagnostic performance of nine commercial kits, which showed optimal performance and high discriminatory power. However, we observed differences in terms of sensitivity, specificity, and E gene Ct Values and discuss these results in light of the influence of SARS-CoV-2 genetic variability and its potential impact in current molecular diagnostic assays.",

author = "Carolina Hern{\'a}ndez and Carolina Florez and Sergio Casta{\~n}eda and Nathalia Ballesteros and David Mart{\'i}nez and Adriana Castillo and Marina Mu{\~n}oz and Sergio Gomez and Angelica Rico and Liseth Pardo and Alberto Paniz-Mondolfi and Ram{\'i}rez, \{Juan David\}",

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doi = "10.1002/jmv.27051",

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AU - Hernández, Carolina

AU - Florez, Carolina

AU - Castañeda, Sergio

AU - Ballesteros, Nathalia

AU - Martínez, David

AU - Castillo, Adriana

AU - Muñoz, Marina

AU - Gomez, Sergio

AU - Rico, Angelica

AU - Pardo, Liseth

AU - Paniz-Mondolfi, Alberto

AU - Ramírez, Juan David

PY - 2021

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AB - The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has led to the design and development of multiple reverse-transcription polymerase chain reaction kits aimed to facilitate the rapid scale-up of molecular testing for massive screening. We evaluated the diagnostic performance of nine commercial kits, which showed optimal performance and high discriminatory power. However, we observed differences in terms of sensitivity, specificity, and E gene Ct Values and discuss these results in light of the influence of SARS-CoV-2 genetic variability and its potential impact in current molecular diagnostic assays.

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DO - 10.1002/jmv.27051

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AN - SCOPUS:85104974684

SN - 0146-6615

JO - Journal of Medical Virology

JF - Journal of Medical Virology

ER -

Evaluation of the diagnostic performance of nine commercial RT-PCR kits for the detection of SARS-CoV-2 in Colombia

Abstract

UN SDGs

All Science Journal Classification (ASJC) codes

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