Effect of therapeutic ultrasound on fibroblast proliferation in vitro

R. P. Cárdenas Sandoval, D. H. Malagón Romero, D. A. Grazón-Alvarado

Research output: Chapter in Book/ReportConference contribution

1 Scopus citations


Fibroblasts are cells that secrete components of the extracellular matrix in fibrillar tissues such as ligaments. During healing ligament process, fibroblasts proliferate and migrate to the injury area in order to produce and remodel collagen type III and type I. Therapeutic physical agents used in physiotherapy are used to improve these processes. Ultrasound is a therapeutic physical agent with high controversy. Experimental studies in humans have not shown effectiveness in the treatment of ligament injuries. In vivo and in vitro studies show discrepancies in their results in terms of cell proliferation rate and production of extracellular matrix components in various soft tissues. However, its use is common in rehabilitation centers. Therefore, the aim of this paper is to describe the effect of ultrasound on cell proliferation. An experimental model in vitro monolayer culture of mouse embryonic fibroblast NIH-3T3 exposed to ultrasound of 1MHz pulsed, 1Watts/cm2 to 50% was used. One of the treatments was exposed to pulsed ultrasound, while the other one, as a control group, was not. The results showed an increase in cell proliferation in the treatment group through the growth curve. In conclusion, this experimental model is the basis for future research about mechanical and biological fibroblasts properties isolated from knee collateral ligaments; it also provides knowledge in cellular levels about effectiveness of ultrasound on ligament healing.

Original languageEnglish (US)
Title of host publicationVI Latin American Congress on Biomedical Engineering, CLAIB 2014
EditorsAriel Braidot, Alejandro Hadad
Number of pages3
ISBN (Electronic)9783319131160
StatePublished - 2015
Externally publishedYes
Event6th Latin American Congress on Biomedical Engineering, CLAIB 2014 - Paraná, Argentina
Duration: Oct 29 2014Oct 31 2014

Publication series

NameIFMBE Proceedings
ISSN (Print)1680-0737


Conference6th Latin American Congress on Biomedical Engineering, CLAIB 2014

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering


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