Striking divergence in toxoplasma ROP16 nucleotide sequences from human and meat samples

Catalina Alvarez, Alejandra De-La-Torre, Mónica Vargas, Claudia Herrera, Lored Dayana Uribe-Huertas, Fabiana Lora, Jorge Enrique Gómez-Marín

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Abstract

Background. ROP16 is a protein kinase of Toxoplasma gondii identified in the mouse model as a virulent marker, but it is unknown whether this finding is relevant in human toxoplasmosis. Methods. We obtained the Toxoplasma ROP16 locus DNA sequence in samples from 12 patients with ocular toxoplasmosis, 1 sample from a patient with congenital toxoplasmosis, 22 samples from soldiers operating in the jungle, 2 samples from urban soldiers, and 10 samples from meat for human consumption. An enzyme-linked immunosorbent assay specific for antibodies against the ROP16 mouse-virulent peptide was performed in 46 serum specimens from patients with ocular toxoplasmosis and in 28 serum specimens from patients with chronic asymptomatic infection, of whom 19 had congenital infection and 11 had toxoplasmic lymphadenitis. Results. We found a striking divergence of the ROP16 nucleotide sequences. Ten of 12 sequences (83.3%) from patients with ocular toxoplasmosis clustered with those of mouse-virulent strains, whereas 7 of 7 ROP16 sequences (100%) from meat were clustered with those of mouse-avirulent strains. Only 11 of 104 serum specimens (10.5%) had specific antibodies against the mouse-virulent peptide, and there was no association between clinical forms and positive results of serological assays. Conclusions. The majority of ROP16 nucleotide sequences from Colombian patients with ocular toxoplasmosis belonged to the group of mouse-virulent strains.
Original languageEnglish (US)
Pages (from-to)2006-2013
Number of pages8
JournalJournal of Infectious Diseases
DOIs
StatePublished - Jan 1 2015

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Toxoplasma
Ocular Toxoplasmosis
Meat
Military Personnel
Serum
Congenital Toxoplasmosis
Lymphadenitis
Peptides
Asymptomatic Infections
Antibodies
Toxoplasmosis
Protein Kinases
Enzyme-Linked Immunosorbent Assay
Infection

Cite this

Alvarez, C., De-La-Torre, A., Vargas, M., Herrera, C., Uribe-Huertas, L. D., Lora, F., & Gómez-Marín, J. E. (2015). Striking divergence in toxoplasma ROP16 nucleotide sequences from human and meat samples. Journal of Infectious Diseases, 2006-2013. https://doi.org/10.1093/infdis/jiu833
Alvarez, Catalina ; De-La-Torre, Alejandra ; Vargas, Mónica ; Herrera, Claudia ; Uribe-Huertas, Lored Dayana ; Lora, Fabiana ; Gómez-Marín, Jorge Enrique. / Striking divergence in toxoplasma ROP16 nucleotide sequences from human and meat samples. In: Journal of Infectious Diseases. 2015 ; pp. 2006-2013.
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abstract = "Background. ROP16 is a protein kinase of Toxoplasma gondii identified in the mouse model as a virulent marker, but it is unknown whether this finding is relevant in human toxoplasmosis. Methods. We obtained the Toxoplasma ROP16 locus DNA sequence in samples from 12 patients with ocular toxoplasmosis, 1 sample from a patient with congenital toxoplasmosis, 22 samples from soldiers operating in the jungle, 2 samples from urban soldiers, and 10 samples from meat for human consumption. An enzyme-linked immunosorbent assay specific for antibodies against the ROP16 mouse-virulent peptide was performed in 46 serum specimens from patients with ocular toxoplasmosis and in 28 serum specimens from patients with chronic asymptomatic infection, of whom 19 had congenital infection and 11 had toxoplasmic lymphadenitis. Results. We found a striking divergence of the ROP16 nucleotide sequences. Ten of 12 sequences (83.3{\%}) from patients with ocular toxoplasmosis clustered with those of mouse-virulent strains, whereas 7 of 7 ROP16 sequences (100{\%}) from meat were clustered with those of mouse-avirulent strains. Only 11 of 104 serum specimens (10.5{\%}) had specific antibodies against the mouse-virulent peptide, and there was no association between clinical forms and positive results of serological assays. Conclusions. The majority of ROP16 nucleotide sequences from Colombian patients with ocular toxoplasmosis belonged to the group of mouse-virulent strains.",
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Striking divergence in toxoplasma ROP16 nucleotide sequences from human and meat samples. / Alvarez, Catalina; De-La-Torre, Alejandra; Vargas, Mónica; Herrera, Claudia; Uribe-Huertas, Lored Dayana; Lora, Fabiana; Gómez-Marín, Jorge Enrique.

In: Journal of Infectious Diseases, 01.01.2015, p. 2006-2013.

Research output: Contribution to journalArticle

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AU - Alvarez, Catalina

AU - De-La-Torre, Alejandra

AU - Vargas, Mónica

AU - Herrera, Claudia

AU - Uribe-Huertas, Lored Dayana

AU - Lora, Fabiana

AU - Gómez-Marín, Jorge Enrique

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N2 - Background. ROP16 is a protein kinase of Toxoplasma gondii identified in the mouse model as a virulent marker, but it is unknown whether this finding is relevant in human toxoplasmosis. Methods. We obtained the Toxoplasma ROP16 locus DNA sequence in samples from 12 patients with ocular toxoplasmosis, 1 sample from a patient with congenital toxoplasmosis, 22 samples from soldiers operating in the jungle, 2 samples from urban soldiers, and 10 samples from meat for human consumption. An enzyme-linked immunosorbent assay specific for antibodies against the ROP16 mouse-virulent peptide was performed in 46 serum specimens from patients with ocular toxoplasmosis and in 28 serum specimens from patients with chronic asymptomatic infection, of whom 19 had congenital infection and 11 had toxoplasmic lymphadenitis. Results. We found a striking divergence of the ROP16 nucleotide sequences. Ten of 12 sequences (83.3%) from patients with ocular toxoplasmosis clustered with those of mouse-virulent strains, whereas 7 of 7 ROP16 sequences (100%) from meat were clustered with those of mouse-avirulent strains. Only 11 of 104 serum specimens (10.5%) had specific antibodies against the mouse-virulent peptide, and there was no association between clinical forms and positive results of serological assays. Conclusions. The majority of ROP16 nucleotide sequences from Colombian patients with ocular toxoplasmosis belonged to the group of mouse-virulent strains.

AB - Background. ROP16 is a protein kinase of Toxoplasma gondii identified in the mouse model as a virulent marker, but it is unknown whether this finding is relevant in human toxoplasmosis. Methods. We obtained the Toxoplasma ROP16 locus DNA sequence in samples from 12 patients with ocular toxoplasmosis, 1 sample from a patient with congenital toxoplasmosis, 22 samples from soldiers operating in the jungle, 2 samples from urban soldiers, and 10 samples from meat for human consumption. An enzyme-linked immunosorbent assay specific for antibodies against the ROP16 mouse-virulent peptide was performed in 46 serum specimens from patients with ocular toxoplasmosis and in 28 serum specimens from patients with chronic asymptomatic infection, of whom 19 had congenital infection and 11 had toxoplasmic lymphadenitis. Results. We found a striking divergence of the ROP16 nucleotide sequences. Ten of 12 sequences (83.3%) from patients with ocular toxoplasmosis clustered with those of mouse-virulent strains, whereas 7 of 7 ROP16 sequences (100%) from meat were clustered with those of mouse-avirulent strains. Only 11 of 104 serum specimens (10.5%) had specific antibodies against the mouse-virulent peptide, and there was no association between clinical forms and positive results of serological assays. Conclusions. The majority of ROP16 nucleotide sequences from Colombian patients with ocular toxoplasmosis belonged to the group of mouse-virulent strains.

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