Development of a multilocus sequence typing scheme for giardia intestinalis

Adriana Higuera, Marina Muñoz, Myriam Consuelo López, Patricia Reyes, Plutarco Urbano, Oswaldo Villalobos, Juan David Ramírez

Research output: Contribution to journalArticlepeer-review

Abstract

Giardia intestinalis is an intestinal protozoan most commonly found in humans. It has been grouped into 8 assemblages (A-H). Markers such as the glutamate dehydrogenase gene, triose phosphate isomerase and beta-giardin (β-giardin) have been widely used for genotyping. In addition, different genetic targets have been proposed as a valuable alternative to assess diversity and genetics of this microorganism. Thus, our objective was to evaluate new markers for the study of the diversity and intra-taxa genetic structure of G intestinalis in silico and in DNA obtained from stool samples. We analysed nine constitutive genes in 80 complete genome sequences and in a group of 24 stool samples from Colombia. Allelic diversity was evaluated by locus and for the concatenated sequence of nine loci that could discriminate up to 53 alleles. Phylogenetic reconstructions allowed us to identify AI, AII and B assemblages. We found evidence of intra-and inter-assemblage recombination events. Population structure analysis showed genetic differentiation among the assemblages analysed.

Original languageEnglish (US)
Article number764
Pages (from-to)1-20
Number of pages20
JournalGenes
Volume11
Issue number7
DOIs
StatePublished - Jul 2020

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

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