TY - JOUR
T1 - Development and validation of an in-house library of colombian candida auris strains with maldi-TOF MS to improve yeast identification
AU - Ceballos-Garzon, Andrés
AU - Amado, Daniela
AU - Vélez, Norida
AU - Jiménez-A, María José
AU - Rodríguez, Crescencio
AU - Parra-Giraldo, Claudia Marcela
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/6
Y1 - 2020/6
N2 - Background: Candida auris is characterized for having a high genetic variability among species. MALDI-TOF MS library contains spectra from only three strains of C. auris, which makes difficult the identification process and gives low scores at the species level. Our aim was to construct and validate an internal library to improve C. auris identification with Colombian clinical strains. Methods: From 30 clinical strains, 770 mass spectra were obtained for the construction of the database. The validation was performed with 300 strains to compare the identification results in the BDAL and C. auris Colombia libraries. Results: Our library allowed a complete, 100% identification of the evaluated strains and a significant improvement in the scores obtained, showing a better performance compared to the Bruker BDAL library. Conclusions: The strengthening of the database is a great opportunity to improve the scoring and C. auris identification. Library data are available via ProteomeXchange with identifier PXD016387.
AB - Background: Candida auris is characterized for having a high genetic variability among species. MALDI-TOF MS library contains spectra from only three strains of C. auris, which makes difficult the identification process and gives low scores at the species level. Our aim was to construct and validate an internal library to improve C. auris identification with Colombian clinical strains. Methods: From 30 clinical strains, 770 mass spectra were obtained for the construction of the database. The validation was performed with 300 strains to compare the identification results in the BDAL and C. auris Colombia libraries. Results: Our library allowed a complete, 100% identification of the evaluated strains and a significant improvement in the scores obtained, showing a better performance compared to the Bruker BDAL library. Conclusions: The strengthening of the database is a great opportunity to improve the scoring and C. auris identification. Library data are available via ProteomeXchange with identifier PXD016387.
UR - http://www.scopus.com/inward/record.url?scp=85086133121&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85086133121&partnerID=8YFLogxK
U2 - 10.3390/jof6020072
DO - 10.3390/jof6020072
M3 - Research Article
AN - SCOPUS:85086133121
SN - 2309-608X
VL - 6
JO - Journal of Fungi
JF - Journal of Fungi
IS - 2
M1 - 72
ER -