TY - JOUR
T1 - Description of Leishmania species among dogs and humans in Colombian Visceral Leishmaniasis outbreaks
AU - Herrera, Giovanny
AU - Higuera, Adriana
AU - Patiño, Luz Helena
AU - Ayala, Martha S.
AU - Ramírez, Juan David
N1 - Funding Information:
We thank the Departamento Administrativo de Ciencia, Tecnología e Innovación ‘COLCIENCIAS’ for funding the Project “Fortalecimiento de la capacidad diagnóstica, de investigación y de vigilancia de enfermedades transmisibles emergentes y reemergentes en Colombia” grant number 757-13 . This work was also funded by DIRECCIÓN DE INVESTIGACIÓN E INNOVACIÓN from Universidad del Rosario.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/10
Y1 - 2018/10
N2 - We report the species detected in dogs and humans from outbreaks of visceral leishmaniasis in Colombia. In this study, 91 sera from patients (n = 38) and dogs (n = 53) diagnosed with visceral leishmaniasis using IFAT were analyzed to determine the causative species. DNA extraction, PCR amplification, DNA sequencing and species identification was performed. Results were obtained with 13 of the sera. A phylogenetic tree and a network of haplotypes were constructed. Leishmania infantum chagasi (11/13), Leishmania braziliensis (1/13) and Leishmania amazonensis (1/13) were identified as the circulating species and genetic variability in one of the L. infantum chagasi strains was demonstrated. This is the first study describing Leishmania species in outbreaks of visceral leishmaniasis in Colombia.
AB - We report the species detected in dogs and humans from outbreaks of visceral leishmaniasis in Colombia. In this study, 91 sera from patients (n = 38) and dogs (n = 53) diagnosed with visceral leishmaniasis using IFAT were analyzed to determine the causative species. DNA extraction, PCR amplification, DNA sequencing and species identification was performed. Results were obtained with 13 of the sera. A phylogenetic tree and a network of haplotypes were constructed. Leishmania infantum chagasi (11/13), Leishmania braziliensis (1/13) and Leishmania amazonensis (1/13) were identified as the circulating species and genetic variability in one of the L. infantum chagasi strains was demonstrated. This is the first study describing Leishmania species in outbreaks of visceral leishmaniasis in Colombia.
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U2 - 10.1016/j.meegid.2018.06.023
DO - 10.1016/j.meegid.2018.06.023
M3 - Article
C2 - 29936036
AN - SCOPUS:85048858386
SN - 1567-1348
VL - 64
SP - 135
EP - 138
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
ER -
