Cutaneous tuberculosis diagnosis in an inhospitable Amazonian region by means of telemedicine and molecular biology.

Diana I. Angel, Rosalba Alfonso, Michel Faizal, Orlando Ricaurte, Julio A. Baez, Andres Rojas, Paola Barato, Manuel E. Patarroyo, Manuel A. Patarroyo

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We report on a 13-year-old boy who displayed a chronic granulomatous inflammatory reaction of 5 years duration. The lesion was resistant to different antibiotic schemes; his routine laboratory tests and chest radiographs were normal. Teledermatologic consultation and histopathologic study of skin biopsy suggested scrofulodermal tuberculosis. Polymerase chain reaction amplification of DNA extracted from lymph node biopsy was taken as starting material for dot-blot hybridization using Mtp-40 and IS 6110 as probes for detecting either Mycobacterium tuberculosis or any mycobacteria belonging to the M tuberculosis complex, respectively. Positive results in both hybridizations were further confirmed by culturing in BACTEC MGIT 960 system. The lesion greatly diminished following isoniazid, rifampin, and ethambutol treatment. Telemedicine allowed a cutaneous tuberculosis diagnosis to be made of a patient living in a remote town located in the Amazon jungle by using molecular biology techniques.
Original languageEnglish (US)
JournalJournal of the American Academy of Dermatology.
StatePublished - May 1 2005
Externally publishedYes

Fingerprint

Dive into the research topics of 'Cutaneous tuberculosis diagnosis in an inhospitable Amazonian region by means of telemedicine and molecular biology.'. Together they form a unique fingerprint.

Cite this