Primary culture of human keratinocytes planted on pig's intestine submucosa

Ángela Ximena Amórtegui, Sandra Rocío Ramírez

    Research output: Contribution to JournalResearch Articlepeer-review

    Abstract

    Tissue engineering, in the fields of skin regeneration, seeks to overcome the limitations associated with the use of immediate auto-grafts, since choosing the donor region of the patient constitutes a risk for the patient himself and is insufficient if the injury that has to be repaired is very large. The use of the pig's small intestine submucosa (SIS) has being proved as a filling biomaterial to treat injuries, because of its special composition, it lowers pain and inflammation since its first application and it favours early mobility to the wounded area. The present study developed a protocol for the primary culture of human keratinocytes from infant foreskins, and used small intestinal submucosa (SIS) like culture substrate. Cellular adherence potential and proliferation capability of the keratinocytes over this substrate was evaluated.

    Translated title of the contributionCultivo primario de queratinocitos humanos sembrados en submucosa intestinal porcina
    Original languageEnglish (US)
    Pages (from-to)8-22
    Number of pages15
    JournalRevista ciencias de la salud
    Volume6
    Issue number3
    StatePublished - 2008

    All Science Journal Classification (ASJC) codes

    • Medicine (miscellaneous)
    • Health(social science)

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