Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides' immunogenicity and in vitro seroneutralisation capability

Catalina Avendaño, Mark Jenkins, Gina Méndez-Callejas, Jairo Oviedo, Fanny Guzmán, Manuel A Patarroyo, Caridad Sánchez-Acedo, Joaquín Quílez

Research output: Contribution to journalArticle

Abstract

Cryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at performing preliminary tests on Swiss albino weaned mice (ICR) to evaluate the humoral immune response induced against peptides derived from Cryptosporidium parvum CP15 (15 kDa sporozoite surface antigen) and CSL (circumsporozoite-like antigen) proteins. Peptides were identified and characterised using bioinformatics tools and were chemically synthesised. The antibody response was determined and the neutralising effect of antibodies was measured in cell culture. Despite all peptides studied here were capable of stimulating antibody production, neutralising antibodies were detected for just two of the CP15-derived ones. Additional studies aimed at evaluating further the potential of such peptides as vaccine candidates are thus recommended.

Original languageEnglish (US)
Pages (from-to)6703-6710
Number of pages8
JournalVaccine
Volume36
Issue number45
DOIs
StateE-pub ahead of print - Sep 26 2018

Cite this

Avendaño, Catalina ; Jenkins, Mark ; Méndez-Callejas, Gina ; Oviedo, Jairo ; Guzmán, Fanny ; Patarroyo, Manuel A ; Sánchez-Acedo, Caridad ; Quílez, Joaquín. / Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides' immunogenicity and in vitro seroneutralisation capability. In: Vaccine. 2018 ; Vol. 36, No. 45. pp. 6703-6710.
@article{8bce74be196a4bc5a70abc68f3fa0b7f,
title = "Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides' immunogenicity and in vitro seroneutralisation capability",
abstract = "Cryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at performing preliminary tests on Swiss albino weaned mice (ICR) to evaluate the humoral immune response induced against peptides derived from Cryptosporidium parvum CP15 (15 kDa sporozoite surface antigen) and CSL (circumsporozoite-like antigen) proteins. Peptides were identified and characterised using bioinformatics tools and were chemically synthesised. The antibody response was determined and the neutralising effect of antibodies was measured in cell culture. Despite all peptides studied here were capable of stimulating antibody production, neutralising antibodies were detected for just two of the CP15-derived ones. Additional studies aimed at evaluating further the potential of such peptides as vaccine candidates are thus recommended.",
author = "Catalina Avenda{\~n}o and Mark Jenkins and Gina M{\'e}ndez-Callejas and Jairo Oviedo and Fanny Guzm{\'a}n and Patarroyo, {Manuel A} and Caridad S{\'a}nchez-Acedo and Joaqu{\'i}n Qu{\'i}lez",
note = "Copyright {\circledC} 2018 Elsevier Ltd. All rights reserved.",
year = "2018",
month = "9",
day = "26",
doi = "10.1016/j.vaccine.2018.09.044",
language = "English (US)",
volume = "36",
pages = "6703--6710",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "45",

}

Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides' immunogenicity and in vitro seroneutralisation capability. / Avendaño, Catalina; Jenkins, Mark; Méndez-Callejas, Gina; Oviedo, Jairo; Guzmán, Fanny; Patarroyo, Manuel A; Sánchez-Acedo, Caridad; Quílez, Joaquín.

In: Vaccine, Vol. 36, No. 45, 26.09.2018, p. 6703-6710.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides' immunogenicity and in vitro seroneutralisation capability

AU - Avendaño, Catalina

AU - Jenkins, Mark

AU - Méndez-Callejas, Gina

AU - Oviedo, Jairo

AU - Guzmán, Fanny

AU - Patarroyo, Manuel A

AU - Sánchez-Acedo, Caridad

AU - Quílez, Joaquín

N1 - Copyright © 2018 Elsevier Ltd. All rights reserved.

PY - 2018/9/26

Y1 - 2018/9/26

N2 - Cryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at performing preliminary tests on Swiss albino weaned mice (ICR) to evaluate the humoral immune response induced against peptides derived from Cryptosporidium parvum CP15 (15 kDa sporozoite surface antigen) and CSL (circumsporozoite-like antigen) proteins. Peptides were identified and characterised using bioinformatics tools and were chemically synthesised. The antibody response was determined and the neutralising effect of antibodies was measured in cell culture. Despite all peptides studied here were capable of stimulating antibody production, neutralising antibodies were detected for just two of the CP15-derived ones. Additional studies aimed at evaluating further the potential of such peptides as vaccine candidates are thus recommended.

AB - Cryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at performing preliminary tests on Swiss albino weaned mice (ICR) to evaluate the humoral immune response induced against peptides derived from Cryptosporidium parvum CP15 (15 kDa sporozoite surface antigen) and CSL (circumsporozoite-like antigen) proteins. Peptides were identified and characterised using bioinformatics tools and were chemically synthesised. The antibody response was determined and the neutralising effect of antibodies was measured in cell culture. Despite all peptides studied here were capable of stimulating antibody production, neutralising antibodies were detected for just two of the CP15-derived ones. Additional studies aimed at evaluating further the potential of such peptides as vaccine candidates are thus recommended.

U2 - 10.1016/j.vaccine.2018.09.044

DO - 10.1016/j.vaccine.2018.09.044

M3 - Article

C2 - 30268732

VL - 36

SP - 6703

EP - 6710

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 45

ER -