Conserved Binding Regions Provide the Clue for Peptide-Based Vaccine Development: A Chemical Perspective.

Hernando Curtidor Castellanos, Cesar Reyes, Adriana Janneth Bermudez Quintero, Magnolia Vanegas Murcia, Yahson Varela, Manuel Elkin Patarroyo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Synthetic peptides have become invaluable biomedical research and medicinal chemistry tools for studying functional roles, i.e., binding or proteolytic activity, naturally-occurring regions’ immunogenicity in proteins and developing therapeutic agents and vaccines. Synthetic peptides can mimic protein sites; their structure and function can be easily modulated by specific amino acid replacement. They have major advantages, i.e., they are cheap, easily-produced and chemically stable, lack infectious and secondary adverse reactions and can induce immune responses via T- and B-cell epitopes. Our group has previously shown that using synthetic peptides and adopting a functional approach has led to identifying Plasmodium falciparum conserved regions binding to host cells. Conserved high activity binding peptides’ (cHABPs) physicochemical, structural and immunological characteristics have been taken into account for properly modifying and converting them into highly immunogenic, protection-inducing peptides (mHABPs) in the experimental Aotus monkey model. This article describes stereo–electron and topochemical characteristics regarding major histocompatibility complex (MHC)-mHABP-T-cell receptor (TCR) complex formation. Some mHABPs in this complex inducing long-lasting, protective immunity have been named immune protection-inducing protein structures (IMPIPS), forming the subunit components in chemically synthesized vaccines. This manuscript summarizes this particular field and adds our recent findings concerning intramolecular interactions (H-bonds or π-interactions) enabling proper IMPIPS structure as well as the peripheral flanking residues (PFR) to stabilize the MHCII-IMPIPS-TCR interaction, aimed at inducing long-lasting, protective immunological memory.
Original languageEnglish (US)
Article number2199
Pages (from-to)1-31
Number of pages31
JournalMolecules
Volume22
Issue number12
DOIs
StatePublished - Dec 22 2017

Cite this

Curtidor Castellanos, H., Reyes, C., Bermudez Quintero, A. J., Vanegas Murcia, M., Varela, Y., & Patarroyo, M. E. (2017). Conserved Binding Regions Provide the Clue for Peptide-Based Vaccine Development: A Chemical Perspective. Molecules, 22(12), 1-31. [2199]. https://doi.org/10.3390/molecules22122199, https://doi.org/10.3390/molecules22122199
Curtidor Castellanos, Hernando ; Reyes, Cesar ; Bermudez Quintero, Adriana Janneth ; Vanegas Murcia, Magnolia ; Varela, Yahson ; Patarroyo, Manuel Elkin. / Conserved Binding Regions Provide the Clue for Peptide-Based Vaccine Development: A Chemical Perspective. In: Molecules. 2017 ; Vol. 22, No. 12. pp. 1-31.
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Curtidor Castellanos, H, Reyes, C, Bermudez Quintero, AJ, Vanegas Murcia, M, Varela, Y & Patarroyo, ME 2017, 'Conserved Binding Regions Provide the Clue for Peptide-Based Vaccine Development: A Chemical Perspective.', Molecules, vol. 22, no. 12, 2199, pp. 1-31. https://doi.org/10.3390/molecules22122199, https://doi.org/10.3390/molecules22122199

Conserved Binding Regions Provide the Clue for Peptide-Based Vaccine Development: A Chemical Perspective. / Curtidor Castellanos, Hernando; Reyes, Cesar; Bermudez Quintero, Adriana Janneth; Vanegas Murcia, Magnolia; Varela, Yahson; Patarroyo, Manuel Elkin.

In: Molecules, Vol. 22, No. 12, 2199, 22.12.2017, p. 1-31.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Conserved Binding Regions Provide the Clue for Peptide-Based Vaccine Development:

T2 - A Chemical Perspective.

AU - Curtidor Castellanos, Hernando

AU - Reyes, Cesar

AU - Bermudez Quintero, Adriana Janneth

AU - Vanegas Murcia, Magnolia

AU - Varela, Yahson

AU - Patarroyo, Manuel Elkin

PY - 2017/12/22

Y1 - 2017/12/22

N2 - Synthetic peptides have become invaluable biomedical research and medicinal chemistry tools for studying functional roles, i.e., binding or proteolytic activity, naturally-occurring regions’ immunogenicity in proteins and developing therapeutic agents and vaccines. Synthetic peptides can mimic protein sites; their structure and function can be easily modulated by specific amino acid replacement. They have major advantages, i.e., they are cheap, easily-produced and chemically stable, lack infectious and secondary adverse reactions and can induce immune responses via T- and B-cell epitopes. Our group has previously shown that using synthetic peptides and adopting a functional approach has led to identifying Plasmodium falciparum conserved regions binding to host cells. Conserved high activity binding peptides’ (cHABPs) physicochemical, structural and immunological characteristics have been taken into account for properly modifying and converting them into highly immunogenic, protection-inducing peptides (mHABPs) in the experimental Aotus monkey model. This article describes stereo–electron and topochemical characteristics regarding major histocompatibility complex (MHC)-mHABP-T-cell receptor (TCR) complex formation. Some mHABPs in this complex inducing long-lasting, protective immunity have been named immune protection-inducing protein structures (IMPIPS), forming the subunit components in chemically synthesized vaccines. This manuscript summarizes this particular field and adds our recent findings concerning intramolecular interactions (H-bonds or π-interactions) enabling proper IMPIPS structure as well as the peripheral flanking residues (PFR) to stabilize the MHCII-IMPIPS-TCR interaction, aimed at inducing long-lasting, protective immunological memory.

AB - Synthetic peptides have become invaluable biomedical research and medicinal chemistry tools for studying functional roles, i.e., binding or proteolytic activity, naturally-occurring regions’ immunogenicity in proteins and developing therapeutic agents and vaccines. Synthetic peptides can mimic protein sites; their structure and function can be easily modulated by specific amino acid replacement. They have major advantages, i.e., they are cheap, easily-produced and chemically stable, lack infectious and secondary adverse reactions and can induce immune responses via T- and B-cell epitopes. Our group has previously shown that using synthetic peptides and adopting a functional approach has led to identifying Plasmodium falciparum conserved regions binding to host cells. Conserved high activity binding peptides’ (cHABPs) physicochemical, structural and immunological characteristics have been taken into account for properly modifying and converting them into highly immunogenic, protection-inducing peptides (mHABPs) in the experimental Aotus monkey model. This article describes stereo–electron and topochemical characteristics regarding major histocompatibility complex (MHC)-mHABP-T-cell receptor (TCR) complex formation. Some mHABPs in this complex inducing long-lasting, protective immunity have been named immune protection-inducing protein structures (IMPIPS), forming the subunit components in chemically synthesized vaccines. This manuscript summarizes this particular field and adds our recent findings concerning intramolecular interactions (H-bonds or π-interactions) enabling proper IMPIPS structure as well as the peripheral flanking residues (PFR) to stabilize the MHCII-IMPIPS-TCR interaction, aimed at inducing long-lasting, protective immunological memory.

U2 - 10.3390/molecules22122199

DO - 10.3390/molecules22122199

M3 - Article

C2 - 29231862

VL - 22

SP - 1

EP - 31

JO - Molecules

JF - Molecules

SN - 1420-3049

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Curtidor Castellanos H, Reyes C, Bermudez Quintero AJ, Vanegas Murcia M, Varela Y, Patarroyo ME. Conserved Binding Regions Provide the Clue for Peptide-Based Vaccine Development: A Chemical Perspective. Molecules. 2017 Dec 22;22(12):1-31. 2199. https://doi.org/10.3390/molecules22122199, https://doi.org/10.3390/molecules22122199