Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption

N N Olaya-Galán, A P Corredor-Figueroa, T C Guzmán-Garzón, K S Ríos-Hernandez, S P Salas-Cárdenas, M A Patarroyo, M F Gutierrez

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leucosis, which has been reported worldwide. BLV has been found recently in human tissue and it could have a significant impact on human health. A possible hypothesis regarding viral entry to humans is through the consumption of infected foodstuffs. This study was aimed at detecting the presence of BLV DNA in raw beef and fresh milk for human consumption. Nested PCR directed at the BLV gag gene (272 bp) was used as a diagnostic test. PCR products were confirmed by Sanger sequencing. Forty-nine per cent of the samples proved positive for the presence of proviral DNA. This is the first study highlighting the presence of the BLV gag gene in meat products for human consumption and confirms the presence of the viral DNA in raw milk, as in previous reports. The presence of viral DNA in food products could suggest that viral particles may also be found. Further studies are needed to confirm the presence of infected viral particles, even though the present findings could represent a first approach to BLV transmission to humans through foodstuff consumption.

Original languageEnglish (US)
Pages (from-to)3125-3130
Number of pages6
JournalEpidemiology and Infection
Volume145
Issue number15
DOIs
StatePublished - Nov 2017

Cite this

Olaya-Galán, N. N., Corredor-Figueroa, A. P., Guzmán-Garzón, T. C., Ríos-Hernandez, K. S., Salas-Cárdenas, S. P., Patarroyo, M. A., & Gutierrez, M. F. (2017). Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption. Epidemiology and Infection, 145(15), 3125-3130. https://doi.org/10.1017/S0950268817002229
Olaya-Galán, N N ; Corredor-Figueroa, A P ; Guzmán-Garzón, T C ; Ríos-Hernandez, K S ; Salas-Cárdenas, S P ; Patarroyo, M A ; Gutierrez, M F. / Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption. In: Epidemiology and Infection. 2017 ; Vol. 145, No. 15. pp. 3125-3130.
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abstract = "Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leucosis, which has been reported worldwide. BLV has been found recently in human tissue and it could have a significant impact on human health. A possible hypothesis regarding viral entry to humans is through the consumption of infected foodstuffs. This study was aimed at detecting the presence of BLV DNA in raw beef and fresh milk for human consumption. Nested PCR directed at the BLV gag gene (272 bp) was used as a diagnostic test. PCR products were confirmed by Sanger sequencing. Forty-nine per cent of the samples proved positive for the presence of proviral DNA. This is the first study highlighting the presence of the BLV gag gene in meat products for human consumption and confirms the presence of the viral DNA in raw milk, as in previous reports. The presence of viral DNA in food products could suggest that viral particles may also be found. Further studies are needed to confirm the presence of infected viral particles, even though the present findings could represent a first approach to BLV transmission to humans through foodstuff consumption.",
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Olaya-Galán, NN, Corredor-Figueroa, AP, Guzmán-Garzón, TC, Ríos-Hernandez, KS, Salas-Cárdenas, SP, Patarroyo, MA & Gutierrez, MF 2017, 'Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption', Epidemiology and Infection, vol. 145, no. 15, pp. 3125-3130. https://doi.org/10.1017/S0950268817002229

Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption. / Olaya-Galán, N N; Corredor-Figueroa, A P; Guzmán-Garzón, T C; Ríos-Hernandez, K S; Salas-Cárdenas, S P; Patarroyo, M A; Gutierrez, M F.

In: Epidemiology and Infection, Vol. 145, No. 15, 11.2017, p. 3125-3130.

Research output: Contribution to journalArticle

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AU - Olaya-Galán, N N

AU - Corredor-Figueroa, A P

AU - Guzmán-Garzón, T C

AU - Ríos-Hernandez, K S

AU - Salas-Cárdenas, S P

AU - Patarroyo, M A

AU - Gutierrez, M F

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AB - Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leucosis, which has been reported worldwide. BLV has been found recently in human tissue and it could have a significant impact on human health. A possible hypothesis regarding viral entry to humans is through the consumption of infected foodstuffs. This study was aimed at detecting the presence of BLV DNA in raw beef and fresh milk for human consumption. Nested PCR directed at the BLV gag gene (272 bp) was used as a diagnostic test. PCR products were confirmed by Sanger sequencing. Forty-nine per cent of the samples proved positive for the presence of proviral DNA. This is the first study highlighting the presence of the BLV gag gene in meat products for human consumption and confirms the presence of the viral DNA in raw milk, as in previous reports. The presence of viral DNA in food products could suggest that viral particles may also be found. Further studies are needed to confirm the presence of infected viral particles, even though the present findings could represent a first approach to BLV transmission to humans through foodstuff consumption.

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Olaya-Galán NN, Corredor-Figueroa AP, Guzmán-Garzón TC, Ríos-Hernandez KS, Salas-Cárdenas SP, Patarroyo MA et al. Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption. Epidemiology and Infection. 2017 Nov;145(15):3125-3130. https://doi.org/10.1017/S0950268817002229