Abstract
Cannabidiol (CBD), a phytocannabinoid from the Cannabis sativa plant, has potential neuroprotective properties. The use of the nematode Caenorhabditis elegans (C. elegans) is a preclinical in vivo model that allows screening of promising molecules for the treatment and study of neurodegenerative diseases such as Parkinson's disease (PD). Alpha synuclein is a protein involved in synaptic vesicle trafficking, which is altered and is part of Lewy bodies in PD. C. elegans strain NL5901 expresses human alpha-synuclein, which allows us to evaluate in vivo changes in this protein. To evaluate the effect of cannabidiol in C. elegans strain NL5901. Objectives: To determine the effect of cannabidiol on the longevity of the C. elegans strains included in the study. To describe the effect of cannabidiol on α-synuclein aggregates in the transgenic C. elegans strain. CBD treatment significantly increased longevity in strain NL5901 from 23 days (CI 0.1-1.1) for controls to 58 days (CI 0.8-7.5) for those receiving 0.3µM CBD. CBD at 3µM increased longevity to 40 days (CI 0.6-5.2), while CBD at 30µM increased it to 50 days (CI 0.8-5.8). Likewise, alpha synuclein aggregates were significantly decreased compared to the control group (mean IF 88.84 ± 38.46) vs 0.3 µM (IF 32.56 ± 12.25), vs 3 µM (IF 36.32 ± 11.36), vs 30 µM ( IF 24.89 ± 6.6). In conclusion, CBD treatment in NL5901 nematodes showed increased longevity and decreased alpha synuclein aggregation in vivo.
Translated title of the contribution | Increased longevity and reduced alpha-synuclein aggregation in cannabidiol-treated Caenorhabditis elegans strain NL5901. |
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Original language | Spanish (Colombia) |
Pages | 1 |
Number of pages | 1 |
State | Submitted - Nov 18 2022 |
Event | 20°vo Día de la Investigación. Fundación Cardio Infantil.: Dos décadas generando conocimiento. - Fundación Cardio Infantil, Bogotá, Colombia Duration: Nov 17 2022 → Nov 18 2022 https://cardioinfantil.org/eventos/20-dia-de-la-investigacion/ |
Conference
Conference | 20°vo Día de la Investigación. Fundación Cardio Infantil. |
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Country/Territory | Colombia |
City | Bogotá |
Period | 11/17/22 → 11/18/22 |
Internet address |
All Science Journal Classification (ASJC) codes
- Neuroscience (miscellaneous)