Analysis of DC-SIGN (CD209) Functional Variants in Patients with Tuberculosis

Luis M. Gómez, Juan Manuel Anaya, Elena Sierra-Filardi, Jose Cadena, Ángel Corbí, Javier Martín

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Abstract

Several lines of evidence suggest that host genetic factors controlling the immune response influence infection by Mycobacterium tuberculosis. Recently, DC-SIGN has been shown to be the major M. tuberculosis receptor on dendritic cells (DCs). The aim of this study was to investigate the influence of DC-SIGN functional polymorphisms -336G/A SNP in the promoter region and insertion/deletion in the "neck" region on the predisposition to tuberculosis. We performed an association study in 110 HIV-negative tuberculosis patients and 299 matched controls. In addition, a total of 155 healthy controls were screened for the tuberculin skin test (TST). DC-SIGN -336 SNP detection was performed by the real-time polymerase chain reaction technology, using the TaqMan 5′ allele. The insertion/deletion in the "neck" region was analyzed by polymerase chain reaction with specific primers. Although an increased frequency of the G allele in tuberculosis patients (23%), as compared with controls (19%), was observed, differences were not statistically significant (OR = 1.31, 95% CI = 0.89-1.94, P = 0.14). On the other hand, DC-SIGN repeat polymorphism in the "neck" region had a very low frequency in the analyzed population. We conclude that the studied polymorphisms are not relevant risk factors for developing tuberculosis in Northwestern Colombian individuals. © 2006 American Society for Histocompatibility and Immunogenetics.
Original languageEnglish (US)
Pages (from-to)808-811
Number of pages4
JournalHuman Immunology
DOIs
StatePublished - Oct 1 2006

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Dendritic Cells
Tuberculosis
Neck
Mycobacterium tuberculosis
Single Nucleotide Polymorphism
Tuberculin Test
Immunologic Factors
Skin Tests
Genetic Promoter Regions
Gene Frequency
Real-Time Polymerase Chain Reaction
Alleles
HIV
Technology
Polymerase Chain Reaction
Infection
Population

Cite this

Gómez, Luis M. ; Anaya, Juan Manuel ; Sierra-Filardi, Elena ; Cadena, Jose ; Corbí, Ángel ; Martín, Javier. / Analysis of DC-SIGN (CD209) Functional Variants in Patients with Tuberculosis. In: Human Immunology. 2006 ; pp. 808-811.
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abstract = "Several lines of evidence suggest that host genetic factors controlling the immune response influence infection by Mycobacterium tuberculosis. Recently, DC-SIGN has been shown to be the major M. tuberculosis receptor on dendritic cells (DCs). The aim of this study was to investigate the influence of DC-SIGN functional polymorphisms -336G/A SNP in the promoter region and insertion/deletion in the {"}neck{"} region on the predisposition to tuberculosis. We performed an association study in 110 HIV-negative tuberculosis patients and 299 matched controls. In addition, a total of 155 healthy controls were screened for the tuberculin skin test (TST). DC-SIGN -336 SNP detection was performed by the real-time polymerase chain reaction technology, using the TaqMan 5′ allele. The insertion/deletion in the {"}neck{"} region was analyzed by polymerase chain reaction with specific primers. Although an increased frequency of the G allele in tuberculosis patients (23{\%}), as compared with controls (19{\%}), was observed, differences were not statistically significant (OR = 1.31, 95{\%} CI = 0.89-1.94, P = 0.14). On the other hand, DC-SIGN repeat polymorphism in the {"}neck{"} region had a very low frequency in the analyzed population. We conclude that the studied polymorphisms are not relevant risk factors for developing tuberculosis in Northwestern Colombian individuals. {\circledC} 2006 American Society for Histocompatibility and Immunogenetics.",
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Analysis of DC-SIGN (CD209) Functional Variants in Patients with Tuberculosis. / Gómez, Luis M.; Anaya, Juan Manuel; Sierra-Filardi, Elena; Cadena, Jose; Corbí, Ángel; Martín, Javier.

In: Human Immunology, 01.10.2006, p. 808-811.

Research output: Contribution to journalArticle

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