A rapid and sensitive assay for histone acetyl-transferase activity

S. Ait-Si-Ali, S. Ramirez, P. Robin, D. Trouche, A. Harel-Bellan

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Histone acetyl-transferases (HATs) seem to be key elements in the regulation of transcription. We have designed an enzymatic assay to quantify HAT enzymatic activity. In this assay, the substrate is a peptide corresponding to the 24 first amino acids of histone H4 which is coupled to biotin. After acetylation using [14C]acetyl-CoA, the peptide is purified on streptavidin beads and the associated radioactivity is measured. This assay is sensitive, rapid and convenient.

Original languageEnglish (US)
Pages (from-to)3869-3870
Number of pages2
JournalNucleic Acids Research
Volume26
Issue number16
DOIs
StatePublished - Aug 15 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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