A proposal for a standardised protocol to barcode all land plants

Mark W. Chase, Robyn S. Cowan, Peter M. Hollingsworth, Cassio Van Den Berg, Santiago Madriñán, Gitte Petersen, Ole Seberg, Tina Jørgsensen, Kenneth M. Cameron, Mark Carine, Niklas Pedersen, Terry A.J. Hedderson, Ferozah Conrad, Gerardo A. Salazar, James E. Richardson, Michelle L. Hollingsworth, Timothy G. Barraclough, Laura Kelly, Mike Wilkinson

Research output: Contribution to journalReview articlepeer-review

491 Scopus citations


We propose in this paper to use three regions of plastid DNA as a standard protocol for barcoding all land plants. We review the other markers that have been proposed and discuss their advantages and disadvantages. The low levels of variation in plastid DNA make three regions necessary; there are no plastid regions, coding or non-coding, that evolve as rapidly as mitochondrial DNA generally does in animals. We outline two, three-region options, (1) rpoC1, rpoB and matK or (2) rpoC1, matK andpsbA-trnH as viable markers for land plant barcoding.

Original languageEnglish (US)
Pages (from-to)295-299
Number of pages5
Issue number2
StatePublished - May 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science


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