Actividad de la β-galactosidasa como marcador de senescencia en cultivos primarios del epitelio superficial del ovario

Translated title of the contribution: β-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium

Lilian Chuaire-Noack, Sandra Rondón-Lagos, Magda Sánchez-Corredor, Milcíades Ibáñez-Pinilla, Sandra Ramírez-Clavijo

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

β-galactosidase activity reflects the rate of cellular aging in vitro. Such activity was quantified at pH 6 in ovarian epithelial cells from 28 donors without a history of cancer, by the chemoluminiscent method. The cells were serially cultured until they achieved the state of permanent growth arrest. During the exponential growth phase, all cultures showed a similar pattern of growth and low β-galactosidase activity. However, both in the onset of decrease replicative activity, as well as in the onset of the stationary phase, there was a significant rise in the enzyme activity. Our results showed that β-galactosidase activity can be considered as a replicative senescence marker of the ovarian surface epithelium at pH 6.

Translated title of the contributionβ-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium
Original languageSpanish
Pages (from-to)351-367
Number of pages17
JournalInvestigacion Clinica
Volume51
Issue number3
StatePublished - Sep 2010

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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