β-galactosidase activity reflects the rate of cellular aging in vitro. Such activity was quantified at pH 6 in ovarian epithelial cells from 28 donors without a history of cancer, by the chemoluminiscent method. The cells were serially cultured until they achieved the state of permanent growth arrest. During the exponential growth phase, all cultures showed a similar pattern of growth and low β-galactosidase activity. However, both in the onset of decrease replicative activity, as well as in the onset of the stationary phase, there was a significant rise in the enzyme activity. Our results showed that β-galactosidase activity can be considered as a replicative senescence marker of the ovarian surface epithelium at pH 6.
|Translated title of the contribution||β-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium|
|Number of pages||17|
|State||Published - Sep 2010|
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