Actividad de la ß-galactosidasa como marcador de senescencia en cultivos primarios del epitelio superficial del ovario.

Translated title of the contribution: β-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium

Magda Carolina Sanchez Corredor, S.R. Ramírez, Sandra Rondón-Lagos, Lilian Chuaire Noack, Milcíades Ibáñez-Pinilla

Research output: Contribution to journalArticlepeer-review

Abstract

ß-galactosidase activity reflects the rate of cellular aging in vitro. Such activity was quantified at pH 6 in ovarian epithelial cells from 28 donors without a history of cancer, by the chemoluminiscent method. The cells were serially cultured until they achieved the state of permanent growth arrest. During the exponential growth phase, all cultures showed a similar pattern of growth and low ß-galactosidase activity. However, both in the onset of decrease replicative activity, as well as in the onset of the stationary phase, there was a significant rise in the enzyme activity. Our results showed that ß-galactosidase activity can be considered as a replicative senescence marker of the ovarian surface epithelium at pH 6.
Translated title of the contributionβ-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium
Original languageSpanish (Colombia)
Pages (from-to)351 - 367
Number of pages16
JournalInvestigacion Clinica
Volume51
Issue number3
StatePublished - Sep 2010

Fingerprint Dive into the research topics of 'β-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium'. Together they form a unique fingerprint.

Cite this