Nanotechnology applied to tumor sensitization: An approach to nanomedicine.

General objective

To evaluate the potential effect of hypoxia on the radiosensitizing capacity of gadolinium-doped carbon dots in in vitro models of cell lines U87, HT29 and primary cultures of cells from glial tumors.

Specific objectives

• Establish the concentration range of the isolated and gadolinium-doped carbon spots that ensures cell viability when subjecting the in vitro models of cell lines U87, HT29 and primary cultures of cells from glial tumors to hypoxia.
• To quantify the change in cell survival generated by exposure to a dose of 6 Grays of ionizing radiation through a medical linear accelerator with the use of isolated carbon doped and gadolinium doped, in tumor tissues under hypoxic conditions.
• Establish the DNA damage generated by exposure to a dose of 6 Grays of ionizing radiation with the use of isolated carbon nanoparticles doped with gadolinium, in tumor tissues under normoxia and hypoxia conditions.