Evaluation of Pathogenicity Related to Resistance and Biofilm Production in Candida spp. Clinical Strains in Cases of Recurrent Vulvovaginal Candidiasis.

  • Pinilla, Gladys, (PI)
  • Muñoz, Liliana, (CoI)
  • Navarrete, Jeannette, (CoI)
  • Munoz Henao, Julian Esteban (CoI)
  • Parra-Giraldo, Claudia M., Universidad Javeriana, (CoI)

Project: Research project

Description

Vulvovaginal candidiasis (VVC) is an infection of the genital mucosa caused by different species of the genus Candida. The objective of this study is to evaluate the pathogenicity of Candida spp associated with the production of iopilm and resistance to antifungals such as fluconazole, itraconazole, miconazole and nystatin in the cervical vaginal mucosa of women with recurrent candidiasis in two medical centers in the city of Bogotá D.C. The samples will be collected by a clinical gynecologist from the Clínica de la Mujer and the Hospital Universitario San Ignacio and identified by mass spectrometry (MALDI-TOF). The susceptibility analysis will be performed using the technique of Minimum Inhibitory Concentration (MIC) by microtitration according to the CLSI M27-A2 protocol, to determine and relate the capacity of biofilm formation with the virulence of the strains will be performed the analysis of Biofilm formation with the XTT reagent, after that we will analyze the expression of genes involved in biofilm synthesis. With these analyses we will be able to correlate the biofilm production capacity with the resistance and virulence of the different clinical strains obtained from women with recurrent candidiasis.
StatusActive
Effective start/end date1/2/181/9/20