Effect of low-level laser therapy for the recovery of ligamentous injuries in fibroblasts isolated from human ligation in young and elderly population: In vitro study.

Introduction: Injury to the ligaments triggers the action of fibroblasts, cells that will proliferate and migrate to the area of injury to initiate the production of the new extracellular matrix such as collagen, a process that is altered during aging.

It has been shown that the application of physical agents such as low-level laser can stimulate the repair function of cells.

However, there is little research on human interarticular ligament fibroblasts that determines the effect of laser therapy in relation to the ligament healing process at different stages of the life cycle.

Objective: To evaluate the biological effect in terms of the expression of type I collagen, viability and proliferation with comparative doses of low-level laser therapy on the healing processes of fibroblasts isolated from human ligament in young and elderly population in a system in vitro culture.

Methodology: Through an in vitro monolayer culture system, the effect of two doses of low-level laser therapy on the biological response of ligament fibroblasts will be evaluated: 1) viability 2) cell proliferation and 3) type I collagen expression , using a colorimetric technique and another immunoassay (ELISA).

Expected results: Description and comparison of the viability, cell proliferation and type I collagen production of ligament fibroblasts irradiated with two doses of low-level laser therapy derived from young people and older adults.

This exploratory study will allow formulating future research on low-level laser therapeutic interventions in human ligament repair.

Injury to the ligaments triggers the action of specialized cells (fibroblasts) which will divide and travel to the area of injury to initiate the formation of new tissue, producing resistant proteins such as collagen.

This process is altered during aging.

It has been shown that laser therapy used in physical therapy can improve the function of these specialized cells.

However, there is little research in this regard.

Therefore, this work evaluates the effect of laser therapy on the function of these specialized cells (fibroblasts) in relation to the stages of ligament repair.

This exploratory study will allow formulating future research on low-level laser therapeutic interventions in human ligament repair.

Low-level laser therapy, fibroblasts, anterior cruciate ligament, wound healing, primary cell culture, cell viability, cell proliferation, type I collagen.