Project Details
Description
This project will develop an innovative method based on MALDI-TOF MS for the rapid detection (less than 6 hours) of antifungal resistance in Candida spp., integrating proteomic and genomic analyses. Over 18 months, 30 clinical strains of species such as C. albicans, C. glabrata, C. parapsilosis, and C. auris will be studied, evaluating their response to fluconazole, caspofungin manogepix, and ibrexafungerp. The methodology will combine the optimization of culture media with isotopically labeled amino acids, proteomic analysis using MALDI-TOF MS with triplicate validation, and sequencing of key genes to correlate resistance mechanisms.
The results will enable the standardization of an accessible protocol that can be easily integrated into clinical laboratories equipped with the MALDI-TOF MS system, significantly reducing diagnostic time compared to current methods and improving the therapeutic management of invasive fungal infections. The partnership between the Universidad del Rosario and the Shaio Clinic will ensure technology transfer. The expected impacts include scientific advances, educational training, and strengthening of epidemiological surveillance of antifungal resistance in Colombia.
The results will enable the standardization of an accessible protocol that can be easily integrated into clinical laboratories equipped with the MALDI-TOF MS system, significantly reducing diagnostic time compared to current methods and improving the therapeutic management of invasive fungal infections. The partnership between the Universidad del Rosario and the Shaio Clinic will ensure technology transfer. The expected impacts include scientific advances, educational training, and strengthening of epidemiological surveillance of antifungal resistance in Colombia.
Keywords
Antifungal susceptibility, MALDI-TOF MS, Candida spp, resistance.
| Status | Not started |
|---|---|
| Effective start/end date | 11/1/26 → 5/31/27 |
UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This project contributes towards the following SDG(s):
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SDG 9 Industry, Innovation, and Infrastructure
Main Funding Source
- Small Amount
Location
- Región Centro Oriente
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