Biomarkers of susceptibility and effect in car painters exposed to organic solvents

Project: Research project

Description

Introduction: Motor vehicle painters are routinely exposed to organic solvents, agents capable of producing mutagenic and carcinogenic effects. Objective: To characterise population susceptibility and evaluate genotoxic effects due to exposure to organic solvents. Methodology: A cross-sectional study was conducted comparing a group of car painters exposed to organic solvents with an unexposed group. Polymorphisms of CYP2E1 and the presence of micronuclei in lymphocytes were determined. Results: 122 workers participated in the study, 62 who worked in car paint shops exposed to solvents and 60 who were not exposed. In relation to questionnaire Q 16, 32% of those exposed reported symptoms suggestive of neurotoxicity. When CYP2E1 polymorphism genotypes and the characteristics assessed in the micronucleus test were compared between the two groups, no statistically significant differences were found. However, when the frequencies of micronuclei, micronuclei, micronuclei cells and nucleo-plasmic bridges between the allele groups c1c1 and c2c2/c1c2 of the CYP2E1 polymorphism were compared in the exposure group, statistically significant differences were found. Conclusions: This study reaffirms that workers exposed to organic solvents with CYP2E1 polymorphisms, specifically with genotype c1c1, are more likely to present mutations in somatic cells, a condition associated with a greater susceptibility to diseases such as cancer.

Key findings

Results: 122 workers participated in the study, 62 who worked in paint shops of cars exposed to solvents and 60 unexposed. In relation to questionnaire Q 16, 32% of those exposed reported symptoms suggestive of neurotoxicity. When comparing the genotypes of CYP2E1 polymorphism and the characteristics evaluated in the micronucleus test between the two groups, no statistically significant differences were found. However, when the micronucleus, micronucleated cell and nucleoplasmic bridge frequencies were compared between the allele groups c1c1 and c2c2/c1c2 of CYP2E1 polymorphism in the exposed group, statistically significant differences were found.

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Short titleBiomarkers of susceptibility and effect in car painters
StatusFinished
Effective start/end date1/15/1612/20/19